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| AbFRONTIER's staff can clone various DNA fragments in to the vector provided
or authorized by customers. You can save time and money by consigning your
project to AbFRONTIER's experienced handling. We design primers for PCR
amplification of a target DNA sequence of your interest. Upon request, we can
construct cDNA libraries from tissues or cell cultures. Once AbFRONTIER
performs your cloning project, your future order will be processed promptly,
based on your previous vector information, which is kept in confidentiality. |
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| Download Files |
| Gene Cloning |
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| We provide DNA amplification or recombinant protein production service using
DNA cloning. DNA cloning using E.coli is most popular and the least
expensive.
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AbFRONTIER Service Features
Cloning possible up to 4kb
Design consulting available according to the cloning purpose for
the optimal experiment scheme.
- Final output form : DNA
- Purpose of protein use : Antibody manufacturing
(antigen), active protein, etc.
Period: 2-3 weeks
Client Requirements
Sample ; Template DNA > 0.2 ㎍
Protocol
1. Primer preparation
2. PCR
3. Enzyme cutting
4. Insertion
5. Transformation
6. Culture
7. Mini preparation
8. Insertion confirm (Enzyme cutting, Sequencing)
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| Cloning service using restriction enzyme when enzyme sites are identical. |
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AbFRONTIER Service Features
Period: 2 weeks
Client Requirements
Sample ; Template DNA > 0.2㎍
Protocol
1. Vector enzyme cutting
2. Electrophoresis
3. Elute target DNA from agarose gel
4. Insertion
5. Electrophoresis
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| Through the use of DNA cloning, DNA amplification or recombinant (manipulated)
protein can be created. Especially, protein expression system using baculovirus
has the feature for the characteristic of similarity to human protein through
post-modification and the system is less expensive compared to that of
expression system using mammalian protein. |
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AbFRONTIER Service Features
pFastBac vector and competent DH10Bac E.coli cells are
being used.
After Recombinant Bacmid DNA was prepared , transfection is done to
insect cell (Sf9).
Recombinant baculovirus manufacturing
Period: 8-10 weeks
Client Requirements
Sample ; Template DNA > 0.2㎍
Protocol
1. Cloning to baculovirus shuttle vector (pFastBac vector)
2. Transformation to competent DH10Bac E.coli cell
3. Transfection of insect cell (Sf9)
4. Recombinant baculovirus production
5. Titration
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| Cloning service using restriction enzyme when enzyme sites are identical.
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AbFRONTIER Service Features
Period: 2-4 weeks
Client Requirements
Sample ; Template DNA > 0.2㎍
Sample information : Full DNA sequence / Mutation site
Protocol
1. Primer preparation
2. PCR
3. Sequencing
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Preparation service for plasmid isolation within cells after cultivating
bacteria cells |
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AbFRONTIER Service Features
Scale detrimental to culture volume. |
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1 ℓ
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0.5 ℓ
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0.2 ℓ
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| DNA volume |
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1-2.5 ㎎ |
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0.3~0.5 ㎎
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0.02~0.1 ㎎
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Period: 1-2 weeks
Client Requirements
Template DNA > 0.5㎍ or cell stock > 100 ㎕
Protocol
1. Bacteria cell culture
2. Plasmid preparation using a commercial kit
3. Electrophoresis
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Consulting/ordering methods
For mode details regarding our service and
large-scale production,
please call +82-2-3277-4450~1, or e-mail us at :
orders@younginfrontier.com
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