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| AbFRONTIER's selection of bacterial and insect cell protein expression systems
provides choices for your application. Standard service includes
start-to-finish purification by our experienced scientific staff, to meet
customers specific needs. |
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| Protein Purification |
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| Solubility and binding test service available on a small scale to identify the
most optimizing purification conditions for protein purification after large
quantity E.coli culture by evaluating the protein expression and
protein solubility. |
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AbFRONTIER Service Features
Time and cost savings by pre-evaluating the solubility or creating
purification environments after mass-cultivation.
Identifying the most suitable condition is easy because
transformation of four different kinds of E.coli is done when provided
with DNA sample.
Period: 2-3 weeks
Client Requirements
- DNA > 0.5 ㎍ or cell stock > 100 ㎕
- Client's test results (More prompt service is possible when the
result is provided)
Protocol
1. DNA transformation (when necessary)
2. E.coli culture 20 ㎖
3. Solubility test
i. Cell harvest
ii. Cell disruption : binding buffer by the cell lysis,
sonication and centrifugation
iii. Electrophoresis : Samples before supernatant and
centrifugation
iv. When soluble, conduct binding test; for his-tagged
protein of the insoluble,
conduct binding tests using denatured form (using
urea).
4. Binding test
i. Tagged protein : Affinity column chromatography
ii. Intact protein : Ion exchange chromatography
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| As baculovirus expression system, solubility and binding test service available
on a small scale to identify the most optimizing purification conditions for
mass protein purification by evaluating the protein expression and protein
solubility. |
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AbFRONTIER Service Features
Time and cost savings possible because purification can be made
easier after mass-cultivation,
or by checking if purification can be done at all.
Period: 2-3 weeks
Client Requirements
Baculovirus stock > 1x 107 pfu/㎖ x 3 ㎖
Protocol
1. Baculovirus preparation
2. Infection of insect cells (High Five cells)
3. Solubility test
i. Cell harvest
ii. Cell disruption : binding buffer by the cell lysis,
sonication and centrifugation
iii. Electrophoresis : Samples before Supernatant and
centrifugation
Electrophoresis : Samples before Supernatant and
centrifugation
iv. When soluble, conduct binding test; for his-tagged
protein of the insoluble,
conduct binding tests using the denatured form (using
urea).
4. Binding test
i. Tagged protein : Affinity column chromatography
ii. Intact protein : Ion exchange chromatography
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| E.coli culture service on a liter scale to amplify the protein
expression from E.coli. |
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AbFRONTIER Service Features
Period: 1 week
Client Requirements
Cell stock > 100 ㎕
Cultivation condition (Our database will be utilized when serviced
with our Optimization Service.)
Protocol
1. Inoculate culture media with a E.coli
2. Culture
3. Cell harvest
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| High Five cell cultivation service on a liter scale to amplify the protein
isolated from the High Five cells infected with recombinant baculovirus.
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AbFRONTIER Service Features
Period: 1 week
Client Requirements
Baculovirus stock > 1x 107 pfu/㎖ x 3 ㎖
Cultivation condition (Our database will be utilized when serviced
with our Optimization Service.)
Protocol
1. Baculovirus preparation
2. Infection of insect cell (High Five cell)
3. Culture
4. Cell harvest
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| Protein purification service of 90% or over in purity for the protein of
interest from a cell. |
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AbFRONTIER Service Features
Capable of purification for tagged protein and intact protein.
90% purity of protein guaranteed
Period: 2-3 weeks
Client Requirements
Cell stock with overexpression levels for the protein of interest.
Purification condition (Our database will be utilized when serviced
with our Optimization Service.)
Protocol
1. Cell disruption : binding buffer by the cell lysis, sonication
and centrifugation.
2. Column Chromatography
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Consulting/ordering methods
For mode details regarding our service and
large-scale production,
please call +82-2-3277-4450~1, or e-mail us at :
orders@younginfrontier.com
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